Mason Greenwood, Ben Godfrey, Jesse Lingard, Aaron Ramsdale, James Ward-Prowse, Ollie Watkins, Ben White have all missed out on the final list having originally made the 33-man provisional squad named by Southgate last week.
An underlying problem saw Greenwood ruled out of the Euros earlier on Tuesday, while despite injury concerns for Jordan Henderson, Harry Maguire and Kalvin Phillips, the trio have all made the final squad.
England’s 26-man squad for Euro 2020
Dean Henderson (Manchester United),
Sam Johnstone (West Brom),
Jordan Pickford (Everton).
Trent Alexander-Arnold (Liverpool),
Ben Chilwell (Chelsea),
Conor Coady (Wolves),
Reece James (Chelsea),
Harry Maguire (Manchester United),
Tyrone Mings (Aston Villa),
Luke Shaw (Manchester United),
John Stones (Manchester City),
Kieran Trippier (Atletico Madrid),
Kyle Walker (Manchester City).
Jude Bellingham (Borussia Dortmund),
Jordan Henderson (Liverpool),
Mason Mount (Chelsea),
Kalvin Phillips (Leeds),
Declan Rice (West Ham).
Dominic Calvert-Lewin (Everton),
Phil Foden (Man City),
Jack Grealish (Aston Villa),
Harry Kane (Tottenham),
Marcus Rashford (Manchester United),
Bukayo Saka (Arsenal),
Jadon Sancho (Borussia Dortmund),
Raheem Sterling (Man City).